Non-viral ex vivo genome-editing in mouse bona fide hematopoietic stem cells with CRISPR/Cas9

We conducted two lines of genome-editing experiments of mouse hematopoietic stem cells (HSCs) with the clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein 9 (Cas9). First, to evaluate the genome-editing efficiency in mouse bona fide HSCs, we knocked out in...

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Bibliografiske detaljer
Udgivet i:Mol Ther Methods Clin Dev
Main Authors: Byambaa, Suvd, Uosaki, Hideki, Ohmori, Tsukasa, Hara, Hiromasa, Endo, Hitoshi, Nureki, Osamu, Hanazono, Yutaka
Format: Artigo
Sprog:Inglês
Udgivet: American Society of Gene & Cell Therapy 2021
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Original Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7873578/
https://ncbi.nlm.nih.gov/pubmed/33614821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtm.2021.01.001
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