Label-free characterization of single extracellular vesicles using two-photon fluorescence lifetime imaging microscopy of NAD(P)H

The heterogeneous nature of extracellular vesicles (EVs) creates the need for single EV characterization techniques. However, many common biochemical and functional EV analysis techniques lack single EV resolution. Two-photon fluorescence lifetime imaging microscopy (FLIM) is widely used to function...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Sorrells, Janet E., Martin, Elisabeth M., Aksamitiene, Edita, Mukherjee, Prabuddha, Alex, Aneesh, Chaney, Eric J., Marjanovic, Marina, Boppart, Stephen A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2021
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7870923/
https://ncbi.nlm.nih.gov/pubmed/33558561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-80813-0
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