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Using loop-mediated isothermal amplification for visual detection of porcine parvovirus 7
We developed and optimized a loop-mediated isothermal amplification (LAMP)-based method to detect porcine parvovirus 7 (PPV7). After using three pairs of specific primers to amplify PPV7 isothermally at 62 °C for 40 min, the amplified product was mixed with SYBR Green I, after which the sample turne...
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| Publicado no: | 3 Biotech |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer International Publishing
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7870734/ https://ncbi.nlm.nih.gov/pubmed/33628706 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-020-02623-5 |
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