Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes

The analysis of phagolysosomes within professional phagocytic cells is facilitated by their isolation. Here, we optimized a protocol for the isolation of intact phagolysosomes from macrophages infected with the spores of Aspergillus fumigatus. Purified phagolysosomes allow improved immunostaining, e...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Goldmann, Marie, Schmidt, Franziska, Kyrmizi, Irene, Chamilos, Georgios, Brakhage, Axel A.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
Assuntos:
Protocol
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7868627/
https://ncbi.nlm.nih.gov/pubmed/33598659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100328
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