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A Yeast Chromatin-enriched Fractions Purification Approach, yChEFs, from Saccharomyces cerevisiae

We have adapted a previous procedure and improved an approach that we named yChEFs (yeast Chromatin Enriched Fractions) for purifying chromatin fractions. This methodology allows the easy, reproducible and scalable recovery of proteins associated with chromatin. By using yChEFs, we bypass subcellula...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Bio Protoc
Main Authors: Cuevas-Bermúdez, Abel, Garrido-Godino, Ana I., Gutiérrez-Santiago, Francisco, Navarro, Francisco
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7853979/
https://ncbi.nlm.nih.gov/pubmed/33654706
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3471
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