Fine tuning the glycolytic flux ratio of EP-bifido pathway for mevalonate production by enhancing glucose-6-phosphate dehydrogenase (Zwf) and CRISPRi suppressing 6-phosphofructose kinase (PfkA) in Escherichia coli

BACKGROUND: Natural glycolysis encounters the decarboxylation of glucose partial oxidation product pyruvate into acetyl-CoA, where one-third of the carbon is lost at CO(2). We previously constructed a carbon saving pathway, EP-bifido pathway by combining Embden-Meyerhof-Parnas Pathway, Pentose Phosp...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Li, Ying, Xian, He, Xu, Ya, Zhu, Yuan, Sun, Zhijie, Wang, Qian, Qi, Qingsheng
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2021
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7852082/
https://ncbi.nlm.nih.gov/pubmed/33531004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-021-01526-1
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