Redirecting substrate regioselectivity using engineered ΔN(123)-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens

The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities. Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regio...

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Publicado no:Sci Rep
Main Authors: Benkoulouche, Mounir, Ben Imeddourene, Akli, Barel, Louis-Antoine, Le Heiget, Guillaume, Pizzut, Sandra, Kulyk, Hanna, Bellvert, Floriant, Bozonnet, Sophie, Mulard, Laurence A., Remaud-Siméon, Magali, Moulis, Claire, André, Isabelle
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7844235/
https://ncbi.nlm.nih.gov/pubmed/33510212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-81719-1
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