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Cell-free Reconstitution of the Packaging of Cargo Proteins into Vesicles at the trans Golgi Network

Protein sorting at the trans Golgi network (TGN) plays important roles in targeting newly synthesized proteins to their specific destinations. The aim of this proposal is to reconstitute the packaging of non-Golgi resident cargo proteins into vesicles at the TGN, utilizing rat liver cytosol, semi-in...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Tang, Xiao, Yang, Feng, Guo, Yusong
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842834/
https://ncbi.nlm.nih.gov/pubmed/33659511
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3537
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