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Targeted genetic changes in Candida albicans using transient CRISPR-Cas9 expression

Candida albicans is an opportunistic fungal pathogen responsible for significant disease and mortality. Absent complete mating and other convenient methods, dissection of its virulence factors relies on robust tools to delete, complement, and otherwise modify genes of interest in this diploid organi...

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Détails bibliographiques
Publié dans:Curr Protoc
Auteurs principaux: Huang, Manning Y., Cravener, Max C., Mitchell, Aaron P.
Format: Artigo
Langue:Inglês
Publié: 2021
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842826/
https://ncbi.nlm.nih.gov/pubmed/33491919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpz1.19
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