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Serial Cryomicrotomy of Saccharomyces cerevisiae for Serial Electron Cryotomography

Electron cryotomography (cryo-ET) is an increasingly popular technique to study cellular structures and macromolecules in situ. Due to poor penetration of electrons through thick biological samples, the vitreously frozen samples for cryo-ET need to be thin. For frozen-hydrated cells, such samples ca...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Main Authors: Ng, Cai Tong, Ladinsky, Mark S., Gan, Lu
Formato: Artigo
Idioma:Inglês
Publicado: Bio-Protocol 2020
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842742/
https://ncbi.nlm.nih.gov/pubmed/33659481
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3831
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