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Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in Nicotiana benthamiana

Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein fused to a second reporter. Both fusion proteins a...

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Publicat a:Bio Protoc
Autors principals: Khosla, Aashima, Nelson, David C.
Format: Artigo
Idioma:Inglês
Publicat: Bio-Protocol 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842733/
https://ncbi.nlm.nih.gov/pubmed/33659407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3747
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