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Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography

Exploring the structure and function of protein complexes requires their isolation in the native state–a task that is made challenging when studying labile and/or low abundant complexes. The difficulties in preparing membrane-protein complexes are especially notorious. The cyanobacterium Synechocyst...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Koskela, Minna M., Skotnicová, Petra, Kiss, Éva, Sobotka, Roman
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842675/
https://ncbi.nlm.nih.gov/pubmed/33659289
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3616
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