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Permethylation and Microfractionation of Sulfated Glycans for MS Analysis

Sulfated glycans are barely detectable in routine mass spectrometry (MS)-based glycomic analysis due to ion suppression by the significantly more abundant neutral glycans in the positive ion mode, and sialylated non-sulfated glycans in the negative ion mode, respectively. Nevertheless, the negative...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Yu, Shin-Yi, Snovida, Sergei, Khoo, Kay-Hooi
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842599/
https://ncbi.nlm.nih.gov/pubmed/33659290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3617
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