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Electrophoretic Mobility Shift Assay of in vitro Phosphorylated RNA Polymerase II Carboxyl-terminal Domain Substrates

Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive carboxyl-terminal domain (CTD) of its largest subunit, RP...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Mayfield, Joshua E., Irani, Seema, Zhang, Yan
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842589/
https://ncbi.nlm.nih.gov/pubmed/33659319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3648
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