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Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli

Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the protein to high quality and near homogeneity. Recentl...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Bio Protoc
Prif Awduron: Cupo, Ryan R., Shorter, James
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Bio-Protocol 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842574/
https://ncbi.nlm.nih.gov/pubmed/33659495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3858
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