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Generation and Testing of Fluorescent Adaptable Simple Theranostic (FAST) Proteins

This protocol provides a step-by-step method to create recombinant fluorescent fusion proteins that can be secreted from mammalian cell lines. This builds on many other recombinant protein and fluorescent protein techniques, but is among the first to harness fluorescent fusion proteins secreted dire...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Main Authors: Flies, Andrew S., Darby, Jocelyn M., Murphy, Peter R., Pinfold, Terry L., Patchett, Amanda L., Lennard, Patrick R.
Formato: Artigo
Idioma:Inglês
Publicado: Bio-Protocol 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842535/
https://ncbi.nlm.nih.gov/pubmed/33659348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3696
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