TetR Regulated in vivo Repression Technology to Identify Conditional Gene Silencing in Genetically Engineerable Bacteria Using Vibrio cholerae Murine Infections as Model System

Investigation of bacterial gene regulation upon environmental changes is still a challenging task. For example, Vibrio cholerae, a pathogen of the human gastrointestinal tract, faces diverse transient conditions in different compartments upon oral ingestion. Genetic reporter systems have been demons...

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Veröffentlicht in:Bio Protoc
Hauptverfasser: Zingl, Franz G., Mitterer, Fabian, Thapa, Himadri B., Schild, Stefan
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2020
Schlagworte:
Methods Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842524/
https://ncbi.nlm.nih.gov/pubmed/33659431
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3774
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