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Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes

Polyethylene glycol calcium (PEG-Ca(2+))-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered into protoplasts derived from somatic tissues or calli via PEG...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Toda, Erika, Okamoto, Takashi
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842353/
https://ncbi.nlm.nih.gov/pubmed/33659352
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3681
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