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The ATPase Activity of Escherichia coli Expressed AAA(+)-ATPase Protein
ATPases are the enzymes that breakdown ATP to ADP and release inorganic phosphate (Pi). Here we provide a detailed protocol to determine the ATPase activity of a recombinant AAA(+)-ATPase protein (GENERAL CONTROL NON-REPRESSIBLE-4 [GCN4]) by spectrophotometric absorption at 360 nm to measure the acc...
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| Publicado no: | Bio Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7842304/ https://ncbi.nlm.nih.gov/pubmed/33659369 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3705 |
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