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Isolation and characterization of extracellular vesicles produced by cell lines

Cells produce two broad classes of extracellular vesicles (EVs), exosomes and microvesicles (MVs). Exosomes are 30–150 nm vesicles derived from multivesicular bodies, while MVs are 200–1,000 nm vesicles that pinch off from plasma membranes. Reliable isolation of EVs is crucial to understand their bi...

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Bibliographic Details
Published in:STAR Protoc
Main Authors: Wang, Fangyu, Cerione, Richard A., Antonyak, Marc A.
Format: Artigo
Language:Inglês
Published: Elsevier 2021
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7829267/
https://ncbi.nlm.nih.gov/pubmed/33532740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100295
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