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Gene insertion in Saccharomyces cerevisiae using the CRISPR/Cas9 system
The aim of this work was to rapidly and efficiently insert target DNA sequences into predetermined genomic sites in Saccharomyces cerevisiae. In this study, we designed two technical routes for gene insertion in the S. cerevisiae genome based on the CRISPR/Cas9 system, and a CRISPR array was inserte...
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| Publié dans: | 3 Biotech |
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| Auteurs principaux: | , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Springer International Publishing
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7822977/ https://ncbi.nlm.nih.gov/pubmed/33520576 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-021-02648-4 |
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