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Delivering defined amounts of purified protein with high precision into living cells
Here, we detail a protocol using electroporation to precisely deliver defined amounts of purified protein into CAD cells. This method allows one million cells to be electroporated with protein simultaneously, with high delivery efficiency and low cell death. Further, by circumventing the normal bios...
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| Publicado no: | STAR Protoc |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7817494/ https://ncbi.nlm.nih.gov/pubmed/33511357 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100272 |
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