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Washing with alkaline solutions in protein A purification improves physicochemical properties of monoclonal antibodies

Protein A affinity chromatography has been widely used for both laboratory scale purification and commercial manufacturing of monoclonal antibodies and Fc-fusion proteins. Protein A purification is specific and efficient. However, there still remain several issues to be addressed, such as incomplete...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Imura, Yuichi, Tagawa, Toshiaki, Miyamoto, Yuya, Nonoyama, Satoshi, Sumichika, Hiroshi, Fujino, Yasuhiro, Yamanouchi, Masaya, Miki, Hideo
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7815873/
https://ncbi.nlm.nih.gov/pubmed/33469121
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-81366-6
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