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Accurate bulk quantitation of droplet digital PCR

Droplet digital PCR provides superior accuracy in nucleic acid quantitation. The requirement of microfluidics to generate and analyze the emulsions, however, is a barrier to its adoption, particularly in low resource or clinical settings. Here, we report a novel method to prepare ddPCR droplets by v...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:bioRxiv
Prif Awduron: Sun, Chen, Liu, Leqian, Vasudevan, Harish N., Chang, Kai-Chun, Abate, Adam R.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Cold Spring Harbor Laboratory 2021
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7814815/
https://ncbi.nlm.nih.gov/pubmed/33469578
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/2021.01.13.424628
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