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Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorti...
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| Publicado no: | STAR Protoc |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7811054/ https://ncbi.nlm.nih.gov/pubmed/33490991 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100286 |
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