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Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of Caenorhabditis elegans

Standard laboratory culture of Caenorhabditis elegans utilizes solid growth media with a bacterial food source. However, this culture method limits control of food availability and worm population density, factors that impact many life-history traits. Here, we describe liquid-culture protocols for p...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Hibshman, Jonathan D., Webster, Amy K., Baugh, L. Ryan
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7811050/
https://ncbi.nlm.nih.gov/pubmed/33490989
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100276
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