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CROP: a CRISPR/Cas9 guide selection program based on mapping guide variants

The off-target effect, in which DNA cleavage was conducted outside the targeted region, is a major problem which limits the applications of CRISPR/Cas9 genome editing system. CRISPR Off-target Predictor (CROP) is standalone program developed to address this problem by predicting off-target propensit...

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Библиографические подробности
Опубликовано в: :Sci Rep
Главные авторы: Aprilyanto, Victor, Aditama, Redi, Tanjung, Zulfikar Achmad, Utomo, Condro, Liwang, Tony
Формат: Artigo
Язык:Inglês
Опубликовано: Nature Publishing Group UK 2021
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC7811000/
https://ncbi.nlm.nih.gov/pubmed/33452424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-81297-2
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