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Cholesteryl-Conjugated Ribonuclease A Exhibits Enzyme Activity in Aqueous Solution and Resistance to Dimethyl Sulfoxide

[Image: see text] Using bovine pancreatic ribonuclease A (RNase A) and cholesterol, we synthesized cholesteryl-conjugated ribonuclease A (CHRNase A) to evaluate the influence of a conjugated hydrophobic moiety on protein function. Nuclear magnetic resonance and matrix-assisted laser desorption/ioniz...

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Vydáno v:ACS Omega
Hlavní autoři: Katsura, Shinji, Furuishi, Takayuki, Ueda, Haruhisa, Yonemochi, Etsuo
Médium: Artigo
Jazyk:Inglês
Vydáno: American Chemical Society 2021
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7807799/
https://ncbi.nlm.nih.gov/pubmed/33458505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.0c05016
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