Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection

Feline bocavirus 1 (FBoV-1) may be associated with diarrhea in cats. In this study, a SYBR Green I-based quantitative polymerase chain reaction (qPCR) assay was established to detect FBoV-1. The melting curve showed a single melting peak at 83.0 ℃. The results of sensitivity showed that the detectio...

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Bibliographische Detailangaben
Veröffentlicht in:3 Biotech
Hauptverfasser: Wang, Yong, Li, Wei, Guo, Xu, Zhang, Da, Sun, Jianfei, Fu, Ziteng, Liu, Guangqing, Li, Yongdong, Jiang, Shudong
Format: Artigo
Sprache:Inglês
Veröffentlicht: Springer International Publishing 2021
Schlagworte:
Short Reports
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7799429/
https://ncbi.nlm.nih.gov/pubmed/33457175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-020-02577-8
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