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Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This pro...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:STAR Protoc
Main Authors: Toda, Gotaro, Yamauchi, Toshimasa, Kadowaki, Takashi, Ueki, Kohjiro
Format: Artigo
Jezik:Inglês
Izdano: Elsevier 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7797923/
https://ncbi.nlm.nih.gov/pubmed/33458708
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100246
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