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Global mapping of translation initiation sites by TIS profiling in budding yeast

Translation initiation site (TIS) profiling allows for the genome-wide identification of TISs in vivo by exclusively capturing mRNA fragments within ribosomes that have just completed translation initiation. It leverages translation inhibitors, such as harringtonine and lactimidomycin (LTM), that pr...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Hollerer, Ina, Powers, Emily N., Brar, Gloria A.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7797918/
https://ncbi.nlm.nih.gov/pubmed/33458709
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100250
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