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Evaluating cytokine production by flow cytometry using brefeldin A in mice

Characterizing cytokine production in situ is important for properly understanding immunologic responses. Cytokine reporter mice are limited by the need to cross markers into various knockout backgrounds and by availability of reporters of interest. To overcome this, we utilize injection of brefeldi...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Kovacs, Stephen B., Oh, Changhoon, Aachoui, Youssef, Miao, Edward A.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7797915/
https://ncbi.nlm.nih.gov/pubmed/33458706
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100244
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