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Ligand incorporation into protein microcrystals for MicroED by on-grid soaking
A method for soaking ligands into protein microcrystals on TEM grids is presented. Every crystal on the grid is soaked simultaneously using only standard cryoEM vitrification equipment. The method is demonstrated using proteinase K microcrystals soaked with the 5-amino-2,4,6-triodoisophthalic acid (...
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| Publicado no: | Structure |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7796918/ https://ncbi.nlm.nih.gov/pubmed/33007196 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2020.09.003 |
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