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Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation
Alveolar type II cells (AT2) are stem cells of lung epithelium. To study these cells, we developed protocols to isolate primary AT2 and test the effects of potential niche factors in regulating the AT2 progenitor functions. AT2 freshly isolated from mouse lungs are grown in 2D or 3D culture. AT2 are...
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| Publicado no: | STAR Protoc |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7788236/ https://ncbi.nlm.nih.gov/pubmed/33437966 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100241 |
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