Extracellular Production, Characterization, and Engineering of a Polyextremotolerant Subtilisin-Like Protease From Feather-Degrading Thermoactinomyces vulgaris Strain CDF

Here, the gene encoding a subtilisin-like protease (protease Als) was cloned from Thermoactinomyces vulgaris strain CDF and expressed in Escherichia coli. The recombinant enzyme was released into the culture medium of E. coli as a mature form (mAls). Purified mAls displayed optimal activity at 60–70...

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Vydáno v:Front Microbiol
Hlavní autoři: Ding, Yidi, Yang, Yong, Ren, Yuxia, Xia, Jingying, Liu, Feng, Li, Yu, Tang, Xiao-Feng, Tang, Bing
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2020
Témata:
Microbiology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7779483/
https://ncbi.nlm.nih.gov/pubmed/33408708
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2020.605771
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