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Enzymatic Analysis of Yeast Cell Wall-Resident GAPDH and Its Secretion

In yeast, many proteins are found in both the cytoplasmic and extracellular compartments, and consequently it can be difficult to distinguish nonconventional secretion from cellular leakage. Therefore, we monitored the extracellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity of intact...

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Vydáno v:mSphere
Hlavní autoři: Cohen, Michael J., Philippe, Brianne, Lipke, Peter N.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7771233/
https://ncbi.nlm.nih.gov/pubmed/33328349
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.01027-20
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