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Analyzing ATP Utilization by DEAD-Box RNA Helicases Using Kinetic and Equilibrium Methods
DEAD-box proteins (DBPs) couple ATP utilization to conformational rearrangement of RNA. In this chapter, we outline a combination of equilibrium and kinetic methods that have been developed and applied to the analysis of ATP utilization and linked RNA remodeling by DBPs, specifically Escherichia col...
Shranjeno v:
| izdano v: | Methods Enzymol |
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| Main Authors: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2012
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7768905/ https://ncbi.nlm.nih.gov/pubmed/22713314 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-396546-2.00002-4 |
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