Relocation of the attTn7 Transgene Insertion Site in Bacmid DNA Enhances Baculovirus Genome Stability and Recombinant Protein Expression in Insect Cells

Baculovirus expression vectors are successfully used for the commercial production of complex (glyco)proteins in eukaryotic cells. The genome engineering of single-copy baculovirus infectious clones (bacmids) in E. coli has been valuable in the study of baculovirus biology, but bacmids are not yet w...

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發表在:Viruses
Main Authors: Pijlman, Gorben P., Grose, Carissa, Hick, Tessy A. H., Breukink, Herman E., van den Braak, Robin, Abbo, Sandra R., Geertsema, Corinne, van Oers, Monique M., Martens, Dirk E., Esposito, Dominic
格式: Artigo
語言:Inglês
出版: MDPI 2020
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7765880/
https://ncbi.nlm.nih.gov/pubmed/33339324
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v12121448
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