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Heat Shock Improves Random Spore Analysis in Diverse Strains of Saccharomyces cerevisiae

Random spore analysis (RSA) is a classic method in yeast genetics that allows high-throughput purification of recombinant haploid spores following specific crosses. RSA typically involves a number of steps to induce sporulation, purge vegetative cells that fail to sporulate, and disrupt the ascus wa...

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Foilsithe in:Front Genet
Main Authors: Burke, Molly K., McHugh, Kaitlin M., Kutch, Ian C.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Frontiers Media S.A. 2020
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7759604/
https://ncbi.nlm.nih.gov/pubmed/33362858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fgene.2020.597482
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