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Heat Shock Improves Random Spore Analysis in Diverse Strains of Saccharomyces cerevisiae
Random spore analysis (RSA) is a classic method in yeast genetics that allows high-throughput purification of recombinant haploid spores following specific crosses. RSA typically involves a number of steps to induce sporulation, purge vegetative cells that fail to sporulate, and disrupt the ascus wa...
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| Veröffentlicht in: | Front Genet |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Frontiers Media S.A.
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7759604/ https://ncbi.nlm.nih.gov/pubmed/33362858 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fgene.2020.597482 |
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