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A quantitative medium-throughput assay to measure Caenorhabditis elegans development and reproduction

Growth and offspring count are two commonly determined toxicological endpoints for chemical- or gene-induced developmental and reproductive effects in Caenorhabditis elegans. Here, we present a protocol for a 96 h, medium-throughput assay, assessing both endpoints quantitatively within an automated...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Wittkowski, Paul, Violet, Norman, Oelgeschläger, Michael, Schönfelder, Gilbert, Vogl, Silvia
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757729/
https://ncbi.nlm.nih.gov/pubmed/33377116
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100224
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