Microfluidics for single-cell lineage tracking over time to characterize transmission of phenotypes in Saccharomyces cerevisiae

The budding yeast Saccharomyces cerevisiae is an excellent model organism to dissect the maintenance and inheritance of phenotypes due to its asymmetric division. This requires following individual cells over time as they go through divisions to define pedigrees. Here, we provide a detailed protocol...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Bheda, Poonam, Aguilar-Gómez, Diana, Kukhtevich, Igor, Becker, Johannes, Charvin, Gilles, Kirmizis, Antonis, Schneider, Robert
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2020
Assuntos:
Protocol
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757727/
https://ncbi.nlm.nih.gov/pubmed/33377118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100228
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