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Quantitative photoconversion analysis of internal molecular dynamics in stress granules and other membraneless organelles in live cells
Photoconversion enables real-time labeling of protein sub-populations inside living cells, which can then be tracked with submicrometer resolution. Here, we detail the protocol of comparing protein dynamics inside membraneless organelles in live HEK293T cells using a CRISPR-Cas9 PABPC1-Dendra2 marke...
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| Publicado no: | STAR Protoc |
|---|---|
| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757677/ https://ncbi.nlm.nih.gov/pubmed/33377110 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100217 |
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