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Generation and isolation of single cells from mouse brain with mosaic analysis with double markers-induced uniparental chromosome disomy

Mosaic analysis with double markers (MADM) technology enables concomitant fluorescent cell labeling and induction of uniparental chromosome disomy (UPD) with single-cell resolution. In UPD, imprinted genes are either overexpressed 2-fold or are not expressed. Here, the MADM platform is utilized to p...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Laukoter, Susanne, Amberg, Nicole, Pauler, Florian M., Hippenmeyer, Simon
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757670/
https://ncbi.nlm.nih.gov/pubmed/33377108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100215
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