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A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses

We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infection of the...

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Publicado en:STAR Protoc
Autores principales: Farzani, Touraj Aligholipour, Chov, Angela, Herschhorn, Alon
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757661/
https://ncbi.nlm.nih.gov/pubmed/33377103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100209
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