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Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography

Cell-free extract derived from the eggs of the African clawed frog Xenopus laevis is a well-established model system that has been used historically in bulk aliquots. Here, we describe a microfluidic approach for isolating discrete, biologically relevant volumes of cell-free extract, with more expan...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Geisterfer, Zachary M., Oakey, John, Gatlin, Jesse C.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757658/
https://ncbi.nlm.nih.gov/pubmed/33377113
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100221
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