Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen

This protocol describes the comparative proteomic profiling of the spleen of wild type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurements, protei...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:STAR Protoc
Autori principali: Dowling, Paul, Gargan, Stephen, Zweyer, Margit, Henry, Michael, Meleady, Paula, Swandulla, Dieter, Ohlendieck, Kay
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2020
Soggetti:
Protocol
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757555/
https://ncbi.nlm.nih.gov/pubmed/33377090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100196
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