Generation of Casp8(FL122/123GG) Mice Using CRISPR-Cas9 Technology

The purpose of this protocol is to describe the generation of missense mutations in mice using CRISPR-Cas9 technology. The current protocol focuses on the generation of a Casp8(FL122/123GG) missense mutation, but it can be adapted to introduce any missense or nonsense mutation. For complete details...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Pelletier, Stephane, Tummers, Bart, Green, Douglas R.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020
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Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757410/
https://ncbi.nlm.nih.gov/pubmed/33377075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100181
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