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A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates

We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined si...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Petropavlovskiy, Andrey A., Tauro, Michael G., Lajoie, Patrick, Duennwald, Martin L.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757406/
https://ncbi.nlm.nih.gov/pubmed/33377076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100182
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