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Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution

Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs) with Wnt3a beads display non-overlapping H3/H4 patterns. He...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:STAR Protoc
Hauptverfasser: Ma, Binbin, Trieu, Tung-Jui, Habib, Shukry J., Chen, Xin
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757403/
https://ncbi.nlm.nih.gov/pubmed/33377072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100178
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