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Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution

Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs) with Wnt3a beads display non-overlapping H3/H4 patterns. He...

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Publicat a:STAR Protoc
Autors principals: Ma, Binbin, Trieu, Tung-Jui, Habib, Shukry J., Chen, Xin
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757403/
https://ncbi.nlm.nih.gov/pubmed/33377072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100178
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