Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells

This protocol describes the use of CRISPR/Cas9-mediated homology-directed recombination to construct a PAX7-GFP reporter in human pluripotent stem cells (hPSCs). PAX7 is a key transcription factor and regulator of skeletal muscle stem/progenitor cells. We obtained heterozygous knockin reporter cells...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Xi, Haibin, Young, Courtney S., Pyle, April D.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2020
Assuntos:
Protocol
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757361/
https://ncbi.nlm.nih.gov/pubmed/33377052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100158
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